INVESTIGATION / RESEARCH
Comparative Study for Carrot Juice and Selenium Supplement in Many Physiological and Biochemical Parameters in Patients with Rheumatoid Arthritis in Kirkuk City
Wedad Mahmood Lahmood Al-obaidi1, Mohanad Hasan Mahmood Al-Izzi2, Aya Saad yaseen2
Available from: http://dx.doi.org/10.21931/RB/2021.06.04.15
Carrot juice is a critical source of vitamins, selenium, and β-carotene, which is suggested to protect from Rheumatoid Arthritis (RA). The present study aimed to show the effect of carrot juice supplementation compared to selenium tablet supplementation, so our study includes (44) blood samples belonging to young men with RA.
All patients aged (20-45) years, 44 blood samples were obtained before treatment at week (0), twenty-four men with RA were supplied with fresh carrot juice. The other group of 20 patients was given an artificial selenium supplement for 21 days as well, and the results were analyzed.The samples were collected from Kirkuk hospital, and external specialized clinical from October/2019 to September /2020; experimental groups were divided into three groups: Group 1 : (44) men Rheumatoid arthritis (RA) before treatment, Group 2: (24) men with RA+ Carrot juice, Group 3: (20) men with RA +Selenium Tab, We reach to following results: the patients who have RA consumption Carrot juice and patients take up Se tab. show significant decrease respectively in RBCs, WBCs, ERS, and RF compared with the Rheumatoid arthritis group, so as the results show a significant decrease in Leptin, IL-6, C-Reactive Protein, and TNF-α concentrations in comparison with the Rheumatoid arthritis group. In contrast, we found a significant increase in GSH, Selenium concentrations, and VD3 in men with RA administration carrot juice and patients' consumption Se tab. Respectively compared with the RA group and, finally, our finding shows no difference in Ceruloplasmin in experimental groups.
Keywords . Rheumatoid Arthritis, Carrot Juice, antioxidant agents, Selenium,VitD3, and Leptin
Rheumatoid arthritis (RA) is an autoimmune, chronic, and inflammatory disease that affects ~1% of the global population 1. It is characterized by excessive production of inflammatory mediators, including cytokines (IL-1β, IL-6, TNF-α, IL-17), chemokines, and autoantibodies that lead to an exacerbated activation of immune system cells. If not treated promptly and adequately, this disease can lead to irreversible joint damage 2. it has been considered a multifactorial disease resulting from the interaction of genetic, hormonal, and environmental factors that contribute to the loss of immune tolerance 3,4,5. Among the environmental risk factors, the diet has been reported to play an essential role in regulating RA disease activity as specific foods can aggravate or attenuate inflammation 6. Recently, it has been established that nutrition is essential for the proper development of the immune system, which has led to the study of the relationship between both of them 7.
Fruits and vegetables are rich sources of nutrients that contain phytochemicals (also known as bioactive compounds), which are recognized for their nutraceutical effects and health benefits. The cultivated carrot (Daucus carota L.) is one of the most essential vegetable plants globally because of its high yield potential and use as a fresh or processed product8. Although pharmacological treatment of RA patients is currently more effective, not all treatments achieve the reduction of the disease but immune-nutrients in the diet of RA patients could be an alternative to improve some disease conditions such as pain alleviation, reduced count of tender joints, and shortening of the morning stiffness duration, which can also influence on the attenuation of the disease clinical activity 9. The importance of the adequate consumption of a diet rich in micronutrients is that they have a fundamental role in the immune system throughout life. Among the micronutrients, vitamins A, C, D, E, and B12 and minerals such as iron, zinc, and selenium are involved in activating and functioning the immune response 10,11.
Figure 1. Rheumatoid arthritis (RA) is an autoimmune, chronic, and inflammatory disease. It is characterized by excessive production of inflammatory mediators, including cytokines (IL-1β, IL-6, TNF-α, IL-17), chemokines, and autoantibodies that lead to an exacerbated activation of immune system cells
Selenium (Se) is an essential micronutrient that is important for various aspects of human health, including proper thyroid hormone metabolism, cardiovascular health, prevention of neurodegeneration and cancer, and optimal immune responses. Very low (depleted) or very high (toxic) levels of Se intake can be detrimental or possibly fatal. Extreme deficiency or toxicity is not commonly found in humans, but selenosis has been reported in cases of miscalculated supplement formulations, suicides, accidental overdose, or intentional poisoning 12. That said, less overt changes in Se status within an individual may still affect inflammation and immune responses. The biological effects of Se are mainly exerted through its incorporation into selenoproteins, and selenoproteins are involved in the activation, proliferation, and differentiation of cells that drive innate and adaptive immune responses. Dietary Se and selenoproteins are not only important for initiating or enhancing immunity but are also involved in immunoregulation, which is crucial for preventing excessive responses that may lead to autoimmunity or chronic inflammation. It should be noted that most studies in the literature involve modifications to dietary Se, and insights into mechanisms often are not clear, but roles for individual selenoproteins and mechanisms are discussed when data are available.
Leptin is a cytokine-like 16 kDa peptide produced mainly through adipose tissue and regulates food intake, basal metabolism, and the β-oxidation of fatty acids. When binding to its receptor(s) located in hypothalamic nuclei occurs, leptin is an essential trigger of adaptive mechanisms during starvation leading to downregulation of thyroid and reproductive functions and stimulation of the hypothalamus. In healthy subjects, leptin levels in the blood are proportional to the body fat mass. Leptin has recently been recognized as a modulator of inflammatory and immune responses. Indeed, leptin participates in bone formation by stimulating osteoblastic cell proliferation and the formation of mineralized nodules in primary osteoblasts and osteosarcoma cells. Leptin facilitates the proliferation of human endothelial cells supporting angiogenesis and neovascularization. Leptin has a dual role in inflammation.
On the one hand, it activates monocyte/macrophage cells and potentiates the production of the pro-inflammatory cytokines, tumor necrosis factor α (TNFα), interleukin (IL)6, and directs T cell differentiation to Th1 phenotype, expressing interferon γ13. Vitamin D is a secosteroid hormone involved in bone and calcium metabolism. It is involved in regulating calcium homeostasis, as it regulates calcium absorption from the gastrointestinal system. The hormone is synthesized in the skin by ultraviolet irradiation Vitamin D has extraskeletal effects as well 14. The non-classical actions of vitamin D are currently under discussion. Vitamin D has been found to have immunomodulatory actions; Vitamin D deficiency has been shown to be correlated with the appearance of autoimmune diseases, such as diabetes mellitus type 1 and multiple sclerosis; Vitamin D deficiency may increase the risk for the development of RA 15. Recently, the role of vitamin D deficiency in the pathogenesis of RA and the relationship between vitamin D deficiency and the activity of RA is discussed. RA is an inflammatory disease characterized by flares and remissions, flares being characterized by pain. Vitamin D deficiency is also known to be associated with diffuse musculoskeletal pain 16.
The current study aims to clarify the effect of Carrot juice administration rich with vitamins, antioxidants, and minerals as selenium to treat Rheumatoid arthritis in men in comparison with artificial selenium supplement main role in increasing the production and release of the antioxidants and minerals and reducing inflammation factor
The study also aims to elucidate the role of natural components for carrots in improving human bone and cartilage abilities through its effect as a natural inducer, dispensing as much as possible with medication or excessive treatment without consulting a doctor. This is because these supplements are an essential natural requirement for the human body in general and particularly for patients in Kirkuk city.
our study include (44) blood samples belonging to young men with RA, we withdraw(44) blood samples before treatment in a week (0), twenty-four men with RA volunteered to consumption fresh carrot juice rich naturally with antioxidants agents and minerals (Fe and Selenium ) for twenty-one days, while the other (20) volunteered has taken selenium tablets as an artificial supplement, With age (20-45) year, the samples were collected from Kirkuk hospital and clinical from October /2019 to September /2020 the samples were given within a specific measurement and a regular schedule at a rate of 250 ml per sample in Every morning for 21 days. experimental groups were divided into three groups: Group 1 : (44) men Rheumatoid arthritis(RA) before treatment, Group 2: (24) men with RA+ Carrot juice, Group 3: (20) men with RA +Selenium Tablet
The beginning fasting was done for the samples for 8 hours, after which blood samples were drawn through the vein in the arm with the use of the tanka to show the vein wall; the blood was placed in Test tubes and placed in the incubator at a temperature of 37 ° for 30 minutes, after which a centrifuge was used Central at a speed of 3000 cycles per minute duration is 15 minutes. Separate the serum from the other ingredients using a micropipette and place it at a temperature of -20 ° C until the Physiological and biochemical tests are performed
Determination of parameters
Estimation total number of RBCs, WBCs, the results were calculated automatically by Hematology Analyzer. In contrast, the results of Erythrocytes sedimentation rate (ESR) calculated by Westergren method is considered the standard method for measuring ESR as a nonspecific indicator of disease activity. Clinicians often use it in assisting the diagnosis and follow-up of many inflammatory disorders.17 Rheumatoid factor (RF)The most commonly used serological method is the latex agglutination test. RF is an IgM class of antibody directed against the Fc portion of the IgG molecule; it is detected by its ability to agglutinate the latex particles coated with the IgG molecule.
Estimation of antioxidant agents -Selenium concentration in blood serum18 estimation Glutathione and Ceruloplasmin, Estimation of VitD3 Concentration in blood serum for Experimental groups: the assay principle combines enzyme immunoassay competition method with a final fluorescent detection ELFA (Enzyme-Linked Fluorescent Assay) technique by using mini vidas device 19.
Estimation of Leptin concentration, IL-6 , C-Reactive Protein and TNF-α level in blood serum.
The one-way analysis of variance (ANOVA) is used to determine whether there are any statistically significant differences between the means of two or more groups. The arithmetic averages of the coefficients were tested using the Dunkin' multiple range test with a level of significance (0.05) to determine the significant differences between the groups.
Effect of consumption Carrot juice in RBCs, WBCS, ESR, and Rheumatoid factor (RF)in Patients with Rheumatoid Arthritis
Our study shows a significant decrease in RBCs count in patients with Rheumatoid arthritis after consuming fresh carrot juice and selenium group 4.0250, 4.5120 respectively in comparison with Rheumatoid arthritis without treatment 6.7962. The results proved that in Table (1) the WBCs was high to 14100 we reduced it to 9171.2, 11660 in groups 2 & 3 So as the ESR also decreased from 43.212 to 29.295, 33.0114 respectively. Our study shows a significant decrease in RF level in patients with Rheumatoid arthritis after consuming fresh carrot juice 6.4011, 6.4482 respectively, compared with the Rheumatoid arthritis group 8.4963.
Table1.The concentration of RBCs, WBCS, ESR, and Rheumatoid factor (RF) in experimental groups.
Effect of consumption Carrot juice in Leptin, IL-6, C-Reactive Protein and TNF-α in Patients with Rheumatoid Arthritis
Our study shows a positive effect for consuming fresh carrot juice in leptin concentration in men suffering from Rheumatoid Arthritis, so our study shows a significant decrease in Leptin level In patients with RA after consuming fresh carrot juice Selenium tab. 23.550, 43.440. Respectively compared with RA with out treatment 56.111, Our study shows a significant decrease in IL-6 concentration in patients with Rheumatoid arthritis after consuming fresh carrot juice and selenium group 2.22 &4.22 respectively compared with Rheumatoid arthritis without treatment 9.67. Our study shows a significant decrease in CRP in patients with Rheumatoid arthritis after consuming fresh carrot juice and selenium group 2.10 & 2.05 respectively compared with Rheumatoid arthritis without treatment 8.73. At the same time, TNF-α concentration witnessed a high decrease in patients with Rheumatoid arthritis after consuming fresh carrot juice and selenium group 20.55& 25.2.77 respectively compared with Rheumatoid arthritis without treatment 48.53.
Table2. The concentration of Leptin, IL-6, C-reactive Protein, and TNF-α in experimental groups.
Effect of consumption Carrot juice in Selenium, Glutathione Ceruloplasmin and VitD3 in Patients with Rheumatoid arthritis
The recent result in our study in table 3 shows a highly significant increase in Selenium (Se) concentration in patients RA +Selenium tablets group, and in RA +carrot juice 86.0021, 66.5400 respectively in compare with Rheumatoid Arthritis without treatment 26.5647 As well as Glutathione(GSH) concentration observed a significant increase in patients with Rheumatoid arthritis consume fresh carrot juice and in RA+ Se tab. Group respectively 3.902, 3.013 as compared with Rheumatoid arthritis without treatment 1.011 So as in VitD3 in patients with Rheumatoid arthritis consume Se tab. And in patients RA +carrot juice 77.0141, 74.2907 respectively as compared with Rheumatoid arthritis without treatment 19.831 While Ceruloplasmin concentration did not have any change in our experimental groups as shown in Tablet 3 in below :
Table3. The concentration of Selenium, Glutathione, Ceruloplasmin, and VitD3, in experimental groups.
Our study found a significant decrease in RBCs, WBCs count in patients with Rheumatoid arthritis after consuming fresh carrot juice; the previous studies showed that autoimmune disorders and infections could result in a high white blood cell count, but a diet rich in vegetables can help reduce its levels reach to normal value White blood cells are important because they fight bacteria and viruses that could cause illness. A reduced count under average level may depress your immunity. A multivitamin, taken in conjunction with plenty of vegetables, can help you get the recommended daily intake of white blood cell-boosting nutrients. On a cellular level, dietary may influence various leukocytic effector functions, including adherence, migration, phagocytosis, and cytokine secretion. Several members of the selenoprotein family regulate or are regulated by cellular redox tone, a crucial modulator of immune cell signaling and function20. So our study agreed with Shweta, and he follows (2017) they found that a fasting of 7–10 days with the partial nutrient intake of vegetable broth, herbal teas, parsley, garlic, and decoction of juice extracts from carrots, celery; and a controlled daily energy intake followed by 1 year of a vegan diet as compared to omnivorous diet was studied in different trials, Together these studies observed a remarkable decrease in swollen and tender joints, pain, erythrocyte sedimentation rate (ESR), and C-reactive protein21.
Dietary selenium (Se), mainly through its incorporation into selenoproteins, plays an essential role in inflammation and immunity. Adequate levels of Se are essential for initiating immunity, but they are also involved in regulating excessive immune responses and chronic inflammation. Evidence has emerged regarding roles for individual selenoproteins in regulating inflammation and immunity, and this has provided important insight into mechanisms by which Se influences these processes. Se deficiency has long been recognized to negatively impact immune cells during activation, differentiation, and proliferation. This is related to increased oxidative stress; RA is a chronic disease requiring long-term intake of drugs, including anti-rheumatics and non-steroidal anti-inflammatory drugs.
Patients with RA are prone to drop out of drug treatment due to the adverse effects. In RA, free radicals are associated with joint inflammation and damage. Antioxidant supplements and diets have long been advocated for the treatment and prevention of RA due to their protective role against free radicals22,it is well-established the role of leptin as a growth factor for the monocytes, promoting phagocytic function and proliferation of circulating monocytes, inducing the production of pro-inflammatory cytokines (TNF-α, IL-6, and IL-12) and stimulating the oxidative burst as well as the chemotactic responses mediating the inflammatory infiltrate. On the other hand, ROS production in HIV-infected patients indicates programmed cell death in monocytes. Even though vitamins and selenium with antioxidant properties have been demonstrated to be beneficial to RA in a cellular study, there are contradicting results concerning the effects of antioxidant vitamins on the development of RA in animal and clinical studies. In the present study, the effects of selenium on the inflammatory cytokine networks were observed. When treated with a diet rich with selenium compared with selenium supplement, the levels of leptin, TNF-α, and IL-6 were significantly reduced in addition to reduce the C-RP and TNF-α levels of were significantly reduced increased. This suggests that vitamins suppress the inflammatory reaction in RA by increasing the levels of anti-inflammatory cytokines and reducing inflammatory cytokines. Due to the complicated cytokine networks in RA, cytokines interact by several signal transduction pathways. Therefore, the simulative effects of different vitamins and trace elements on inflammatory factors levels may be counteracted by the interaction between cytokines in RA23,24.
Our study agreed with the previous study, showing that antioxidant agents were significantly higher in the selenium-treated sheep than in control in the samples taken 14 days after lambing and 30 days after lambing 25,26. Another paper showed that treatments of Selenium supplementation affected an increase in whole blood antioxidant agents and antioxidant enzyme glutathione peroxidase (GSH-Px) as well as increased plasma Selenium(Se) concentrations in experimental groups. On the other hand, the liver Se exhibited a dose-response relationship to treatment, but kidney Se concentrations were unaffected by treatment. Some dietary meat rich with Se concentrations can therefore be increased by supplementation and could contribute to increased human dietary intakes of the element 27, as well as carrot juice good source of carotenoids which act as antioxidants and help detoxify the system because antioxidants fight free radicals and reduce oxidative stress so they help stimulate metabolism 28,29. The data support a role for vitamin D deficiency in the development and progression of autoimmune inflammatory conditions in general, particularly RA. Earlier animal models indicate that the 1,25(OH)2D3 metabolite and its analogs may suppress collagen-induced arthritis. Other data suggest that vitamin D receptor agonists may also prevent and suppress established collagen-induced arthritis; however, data show that vitamin D may be negatively affected in acute response, that is, its levels may decrease in the setting of inflammation, such as inactive RA.30,31,32.
Despite that, treatment with rituximab in RA did not affect vitamin D levels. However, it decreased indices of inflammation Supplementation with selenium induces a level of vitamin D in plasma blood which has been proposed to induce immune tolerance and thus prevent the development of autoimmune diseases33. Recently, the combination of anti-rheumatic and the drugs of dietary supplement of selenium induce and increase the level of vitamin D has been suggested for RA Patients with RA are prone to osteoporosis as well as for its possible effects on disease activity 34,35.
Consumption of natural juice rich with selenium, vitamin D, and several antioxidants agents more beneficial in the treatment of Rheumatoid arthritis than taken artificial supplements should be taken with caution given its possible toxic effects that exceed its recommended consumption limits.
Conflict of Interests
The authors of this paper declare that it has no financial or personal relationships with individuals or organizations that would change unacceptably bias the content of this paper and therefore declare that there is no conflict of interests.
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Received: 26 February 2021
Accepted: 27 July 2021
Wedad Mahmood Lahmood Al-obaidi1, Mohanad Hasan Mahmood Al-Izzi2, Aya Saad yaseen2
1Department of Biology-College of Education-Al-Hawija University of Kirkuk
2Department of Biology- College of Science- University of Tikrit
*Corresponding author email is: [email protected] [email protected], https://orcid.org/0000-0002-2833-0525