2023.08.01.91
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Exodia phenomenon of foodborne Mycophages cocktails against chimeric strains of Candida albicans recovered from dairy chain ecosystems in Baghdad
Ali Al-Shammary 1, and Moutaz Abdul Mounam 2,*
1 Department of Veterinary Public Health / College of Veterinary Medicine / University of Baghdad. Email: [email protected] .
2 Department of Veterinary Public Health / College of Veterinary Medicine / University of Baghdad. Email: [email protected]
* Correspondence: [email protected]
Available from: http://dx.doi.org/10.21931/RB/2023.08.01.91
ABSTRACT
Influential, organized groups with natural antimicrobial and anti-biofilm broad-spectrum power exist within the food chain, like a hidden dormant mimic hygienic bio life nanobodies that can terminate multiple opportunistic disease entities owing multi-stress resistant forbidden recalcitrant power, such as Candida albicans. These wonderful dynamic forces created by ALLAH Almighty are the Mycophages or fungi-eating state of fungi foodborne phages, and this project was redirected to be a dare to leap from us towards the future. Multi-stress resistant C. albicans that are resistant to different antifungal agents with their genetic tolerance plasticity to thermal pasteurization decontamination module as well as to ultraviolet irradiation hurdle strategy recovered from raw milk (mastitis), yogurt and soft cheese with versatile phenotypes resident in topic sectors of Abu-Ghraib, Al-Fudhaliyah and Al-Sadrya in Baghdad. From the other side of trueness, we discover an abnormal deviated activity of bacteriophages cocktails that behave with broad-spectrum functions against Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Streptococci (VRE) as lytic bactericidal and versus multi stress resistant C. albicans as redirected terminator lytic Mycophages thus objected to be a new nano-built hygienic phenomenon entity (Exodia).
Keywords: Exodia, Lytic Mycophages, Multi stress-resistant Candida albicans, dairy chain ecosystems
INTRODUCTION
Global multidrug resistance pandemic problems with massive frequency and distribution patterns can hit and threaten a strike crisis worldwide without any alarm warning. Misuse, inadequate and poor manipulation of antibiotics in medicine and livestock farming can change sensitivity patterns with the natural acquisition of epigenetic tolerance cascaded by genetic resistance. Antibiotics escape sequelae in the pathogenic matrix under selective pressure and terminate with forbidden sequences or adaptive chain of tolerance cascaded by the opposition with the appearance of persisters. Upgraded evolution under elevated concentrations of drugs as natural legacy transformation resident in efflux pumps terminating with resistance aberrant. Shifting to this forbidden recalcitrant behavior due to biofilm entity barrier can dramatically increase with increased exposure to antibiotics or similar shortcuts among and within versatile and diverse environment culmination in resident ecosystem cascaded by priority elevation of a minimum inhibitory concentration of that selected or cross thresholds of antibiotics. Building up these behaviors might exaggerate the pandemic situation by constructing forbidden subclones as persisters phenotype 1,2.
The evolution of bacteriophage matrix ecosystems displayed a pressing issue in developing new diverse, and versatile strategies to overcome and combat emergent and resident biohazard and bioterror infectious foci. Global limitations and obstacles in the massive production of such hygienic nanobodies concern in their efficacy or potency thresholds overwhelmed by CRISPR-CAS resistance module, stability shelf-life, diversity in broad-spectrum viability and action potential, segregation of hygienic lytic arm from forbidden lysogeny, public fearing and an understanding from vital applications, etc. Characterization and redirection of special topics are important features of future projects. The Pharmacokinetics and pharmacodynamics of recovered phage cocktails must be deciphered and illustrated carefully 1,3-5.
Evolution of emergent and reemergent transmissible and zoonotic diseases crosslinked with stress adaptation and stress hardening complex network stimuli that hits and storms with unprecedented or unexpected severity in macro and microbiome, environment, human and animals. Exaggerated and continuous contamination and pollution with forbidden chemical residues cascaded by integral genes sharing behaviors of antibiotics resistance that are vertically and horizontally resident and transmitted within the food chain, population and environment ecosystems can assist dramatically in denominator outbreaks. Establishing, educating and proceeding of knowledge about these sophisticated risks with international hygienic agencies like CDCs and FDA could reduce these emergencies. Besides, new formulations and synthesis of new generations of antimicrobial agents cascaded by the rapid evolution of microbial resistance issues due to quorum sensing behaviors could exaggerate the hygienic situation. The frequency and distribution pattern of such forbidden struggling in food-producing livestock could detrimentally and adversely affect the food chain with expensive processing achievement in man and animals 1,3-5.
Host-specific receptor interaction could promote efficacy for unipotent phage or pluripotent groups. Therefore, each phage or family's specificity and sensitivity required understanding each target's physiology and interactions with the host in selective toxicity caution. Genetically, well-equipped phages display their unique envelope pore-forming units endolysins enzymes with fusion power to target pathogen and then biodegradation of its network. During entry of the phage genome inside the cytoplasmic ecosystem of the target, their segregation ecomap and dependency either proliferate without lysis of target as temperate or proliferate and lyse the target as a lytic module or integrate without increase with the target genome to become prophage entity 1,3-5.
Integrated virions change the genetic ecosystem of the target to become more powerful and stable against invaders, even lytic phages, with the development of target immune barriers such as intelligent clustered regularly interspaced short palindromic repeats (CRISPR) that modulate trusted platform module resident within. Phages are diverse and versatile in their workstation to be important in the biodegradation of toxic chemical residues within the environment. The metagenomic Era of these intelligent functional, unique nanobodies makes them an inexhaustible atomic biofuel. Chainsaw puzzles are present in the dynamic recovery of bacteriophages from wastewater or man or animal residues 1,3-5. but they were literally recovered from the dairy chain in Baghdad 6,7.
During recovery of lytic bacteriophages cocktails from dairy chain against MRSA strains, these recovered phages are redirected to check out their efficacy module against multi-stress resistant C. albicans recovered together with MRSA and VRE from the same resident built-in slime. Broad-spectrum action potential threshold activity displayed for these recovered lytic bacteriophages. Redirected discovered a special topic in this verified project to innovate a new intelligent strategy to overcome these forbidden struggling bioterror entities inside the food chain as a modified regime of hazard analysis critical control points cascaded by Exodia Mycophages against Chimera C. albicans 6,7. Depending on this accumulative knowledge, we decided to build up a designed project aimed, objected and redirected to the hygienic application of recovered bacteriophages against multi-stress resistant chimeric C. albicans.
MATERIALS AND METHODS
Special Topics built-in design
An authenticated modified and verified cascaded series enrollments were dependent for establishing a net framework built-in design 8-18 in which two-hundred and twenty-five pooled samples were collected and verified from sectors of Al-Fudhaliyah, Al-Sadrya and Abu-Ghraib from January to March (2022). An authenticated, modified and verified cascaded series for isolation, identification and confirmation depended on this torment. Cows of raw milk, fresh yogurt, and fresh soft cheese are twenty-five units from each sector for each brand within specified month (a total of seventy-five per episode). Clinical signs with DNA dependent California kit reveal and confirm mastitis. Enrollment of multi-stress resistant C. albicans was verified by C. albicans chrome agar, 16S rRNA PCR, sensitivity pattern to azoles, microtitre plate assay and plasmid Congo red agar for assessment of biofilm, thermotolerance pattern to pasteurization regime and tolerance pattern to ultraviolet irradiation. Freshly prepared Bacteriophages cocktails (MacFarland titer 109 PFU.ml-1) were justified and achieved from consecutive synchronous work on Methicillin-resistant Staphylococcus aureus 6 and Vancomycin-resistant Streptococci (VRE) 7. Chi-square analysis was dependent on the verification of data 19.
RESULTS
Colloquially frequency and distribution pattern unveiled ecomap segregation of four forbidden recovered super isolates of PCR primed C. albicans from two-hundred and twenty-five pooled samples (1.77 %) resident in March with multi-stress recalcitrant biofilm behaviors as two super forbidden isolates (2.66 % and entirely 0.88 %) recovered from raw milk and fresh yogurt resident in Abu-Ghraib, one super forbidden isolate (1.33 % and completely 0.44 %) retrieved from fresh yogurt resident in Al-Fudhaliyah and one super forbidden isolate (1.33 % and entirely 0.44 %) recovered from fresh soft cheese resident in Al-Sadrya. Potent lytic bacteriophages were recovered from fresh yogurt in Abu-Ghraib and verified by plaques forming technique on Muller-Hinton agars against MRSA with scanning electron micrographs and on Vancomycin-resistant Streptococci (VRE). The plaques technique achieved an effective termination module of these forbidden recovered entities throughout droplet and pour plate procedures. Configured tables (Table 1 & Table 2) and photographs decipher and illustrate cascaded events (Figure 1)
A, B: Indicate significant differences vertically among sectors at level (P≤0.05).
a, b: Indicate significant differences horizontally among sectors at level (P≤0.05).
Table 1. Recovery ecomap of segregated PCR primed multi stress resistant C. albicans.
Table 2. Efficacy module of Bacteriophages as Mycophages.
Stress adaptation with phase variation epigenetic tolerance behavior upgraded to stress hardening in genetically well-equipped multi-stress resistant forbidden opportunistic entities within an enclosed electromagnetic environment of biofilm with integrated genes sharing strategies and quorum sensing behaviors in two forbidden biohazards and bioterror recovered resident invaders MRSA, VRE and C. albicans from food chain inside Baghdad ecosystem 6,7. Such struggling forbidden and biohazard entities resident within the food chain in Baghdad were encountered in the previous novel works 20-29.
During recovery of lytic bacteriophages cocktails from dairy chain against MRSA strains, these recovered phages are redirected to check out their efficacy module against multi-stress resistant C. albicans recovered together with MRSA and VRE from the same resident built-in slime. Broad-spectrum action potential threshold activity displayed for these recovered lytic bacteriophages. Redirected discovered particular topic in this verified project to innovate a new intelligent strategy to overcome these forbidden struggling bioterror entities inside the food chain as a modified regime of hazard analysis critical control points cascaded by Exodia Mycophages against Chimera C. albicans 6,7.
Figure 1. Large zones of inhibition of MRSA phages against multi-stress resistant C. albicans on Muller-Hinton agars after 24 hours.
Biodiversity versatile interactions among diverse Eco biota of bacteria, fungi, and viruses within an enclosed microbiome ecosystem are generally called a rotifer entity. Various complex networks reside among and within these denominators to share configured lifestyle behaviors. Because of these rebuilding and remixing machines strategies cascaded by the evolution of forbidden chimeric genes verification module from which resistance problems to different stressors and cross hardening even not encountered else causing programming to reemerge as biohazard terror 28,31.
Frequency and distribution pattern of aggressive C. albicans and Aspergillus forbidden denominators predispose to upsurge indisposition and transience in immune deviated individuals. Resident resistance pattern in these infectious entities remains a problematic issue. To motivate cornerstone therapy, selective verification of new combat strategies in programmed hazard and biohazard analyses critical control points must be transferred. Lytic Mycophages is a novel and unique hygienic design to overcome and combat life-terminating bioterror mycosis. Justified and daring publications must put the segregation benefits for these now-needed weapons. Information about hygienic certification and the application of these nanobodies for therapy and decontamination strategies is still under warranty 31.
Metagenomic verified Era of single or double-stranded RNA or DNA Lytic Mycophages display and segregated their ancestors into more than ten relations or enrollments and over ninety species in dependence of the international committee of viral-based taxonomy. To date, the mainstream of categorized Mycophages own metagenomic double-stranded RNA. These justified Mycophages segregated into four enveloped capsid families: Partitiviridae, Totiviridae, Chrysoviridae, and Reoviridae, with one none, enveloped ancestor, Hypoviridae. Activation of genes responsible for triggering programmed cell suicide arises during heterokaryon between a fungus and lytic phages, while lysogeny arises during incompatible heterokaryon 32.
CONCLUSIONS
Multi-stress resistant C. albicans that are resistant to different antifungal agents with their genetic tolerance plasticity to thermal pasteurization decontamination module as well as to ultraviolet irradiation hurdle strategy recovered from raw milk (mastitis), yogurt and soft cheese with versatile phenotypes resident in topic sectors of Abu-Ghraib, Al-Fudhaliyah and Al-Sadrya in Baghdad. From the other side of trueness, we discover an abnormal deviated activity of bacteriophages cocktails that behave with broad-spectrum functions against Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Streptococci (VRE) as lytic bactericidal and versus multi stress resistant C.
REFERENCES
1. Aldayel, M. F. Biocontrol strategies of antibiotic-resistant, highly pathogenic bacteria and fungi with potential bioterrorism risks: bacteriophage in focus. Journal of King Saud University – Science, 2019, 847: 1-19.
2. Pacios, O.; Blasco, L.; Bleriot, I.; Garcia, L. F.; Bardanca, M. G.; Ambroa, A.; López, M.; Bou, G. and Tomás, M. Strategies to Combat Multidrug-Resistant and Persistent Infectious Diseases. Antibiotics, 2020, 9 (65): 1-20.
3. Lima, R.; Fiol, F. S. D. and Balcao, V. M. Prospects for the Use of New Technologies to Combat Multidrug-Resistant Bacteria. Front. Pharmacol, 2019, 10 (692): 1-10.
4. Garvey, M. Bacteriophages and the One Health Approach to Combat Multidrug Resistance: Is This theWay? Antibiotics, 2020, 9 (414): 1-17.
5. Abdalbakee, H. N. & Mohammed, Th. T. Effect of using different levels of azolla as a substitute for soybean meal in the production performance of fish carp. Plant Archives. 2019, 19(1): 573-577. Doi: 10.13140/RG.2.2.23568.15367.
6. Al-Naseri, J. S. A. and Al-Shammary, A. H. A. Dairy Chain Phages Cocktails against Extended Spectrum Beta Lactamase Resistant Staphylococcus aureus. Plant Archives, 2020b, 20 (2): 21-31.
7. Al-Shammary, A. H. A. and Abdul Mounam, M. A. W. Modern Phage modulation strategy on Vancomycin-Resistant Enterococci (VRE) recovered from mastitis in Baghdad. Biochem. Cell. Arch, 2020, 20 (2): 5433-5442.
8. Quinn, P. J.; Carter, M. E.; Markey, B. and Carter, G. R. Clinical Veterinary Microbiology. 2nd ed., Mosby Int., USA, 2004.
9. M. Ajeel, A.; A. Mehdi, L. . Effect Of Eruca Sativa Seeds Powder As Feed Supplementation On Some Physiological Traits Of Male Lambs. Journal of Life Science and Applied Research. 2020, 1, 20-30..
10. Jay, J. M.; Loessner, M. J. and Golden, D. A. Modern Food Microbiology. 7th. Ed. Springer, 2005.
11. Christensen, G. D., Simpson, W. A., Younger, J. A., Baddour, L. M., Barret, F. F. and Melto, D. M. Adherence of coagulase negative Staphylococci to plastic tissue cultures: a quantitive model for the adherence of Staphylococci to medical devices. J. Clin. Microbiology, 1985, 22: 996-1006.
12. Bauer, A. W.; Kirby, W. M. M.; Sherris, J. C. and Turck M. Antibiotic susceptibility testing by a standardized single disk method. Amer. I. Clin. Pathol, 1966, 45:493-6.
13. Lalitha, M.K. Manual on Antimicrobial Susceptibility Testing, 2004.
14. Clinical and Laboratory Standards Institute (CLSI). Methods for dilution antimicrobial susceptibility testing for bacteria that grew aerobically. Approved Standard M7–A10. Wayne, PA, 2009.
15. Food and Agriculture Organization of the United Nations. Proceedings of the International Workshop on the Use of Antimicrobials in Livestock Production and Antimicrobial Resistance in the Asia-Pacific Region. Food and Agriculture Organization of the United Nations, Rome, Italy, 2012.
16. Etemadifara, Z.; Gholamia, M. and Derikvanda, P. UV-resistant bacteria with multiple-stress tolerance isolated from desert areas in Iran. Geomicrobiology J., 2016, 33 (7): 22-27.
17. Fodor, A.; Abate, B. A.; Deák, P.; László Fodor, L.; Gyenge, E.; Klein, M. G.; Koncz, Z.; Muvevi, J.; Ötvös, L.; Székely, G.; Vozik, D. and Makrai, L. Multidrug Resistance (MDR) and Collateral Sensitivity in Bacteria, with Special Attention to Genetic and Evolutionary Aspects and to the Perspectives of Antimicrobial Peptides—A Review. Pathogens, 2020, 9 (522): 1-53.
18. Grandio, M. L.; Cortesao, M.; Mirete, S.; Camara, M. B.; Figueras, C. G.; Pantoja, D. P.; White, J. J.; Farías, M. E.; Mora, R. R. and Pastor, J. E. G. Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate from the Metagenomes of Hypersaline Environments. Front. Microbiol, 2020, 11 (453): 1-15.
19. SPSS. Statistical Package for the Social Sciences, Version 25, User's guide SPSS Inc., Chicago III, USA. Website http://www.spss.com/, 2019.
20. Al-Shammary, A. H. Detection of Listeria monocytogenes in Milk and Some Dairy Products. PhD Thesis. College of Veterinary medicine, University. of Baghdad, Iraq (In Arabic), 2009.
21. Al-Shammary, A. H. A. and Abdul Mounam, M. A. W. Incidence of Listeria in table-eggs in Baghdad Markets. Interna J Sci Technol, 2011, 6 (2): 93-98.
22. Kanaan, M. H. G. and Al-Shammary, A. H. A. Detection of methicillin or multidrug resistant Staphylococcus aureus (MRSA) in locally produced raw milk and soft cheese in Baghdad markets. The Iraqi Journal of Veterinary Medicine, 2013, 37 (2): 226 -231.
23. Begley, M & Hill C. Stress Adaptation in Foodborne Pathogens. Annu. Rev. Food Sci. Technol, 2015, 6: 9.1–9.20.
24. Al-Shammary, A. H. A. Correlation of Biofilms-Pyocyanin producing Pseudomonas aeruginosa with the antibiotic's resistant profiles A. Internat J for Sci and Technol, 2015a, 10 (1): 83-89.
25. Al-Shammary, A. H. A. Epidemiological Pattern of Extended Spectrum Beta Lactamase Resistant Klebsiella pneumoniae (ESβL) isolated from Mastitic Milk in Baghdad. Curr Res Microbiol Biotechnol, 2017, 5 (4): 1170-1177.
26. Al-Shammary, A. H. A. and Abdul Mounam, M. A. W. The Effect of Oak, Cinnamon, Oregano and Thyme Extracts on Biofilm producing ESβL Klebsiella pneumoniae. IJPS, 2017, 7 (5): 1839-1847.
27. Al-Shammary, A. H. A. Run-off Patterns of Vancomycin Resistant Enterococci (VRE clones) in Cows Raw Milk and Imported Milk Powders at Baghdad Markets. Iraqi J. Vet. Med, 2019, 43 (2): 61 – 66.
28. Al-Naseri, J. S. A. and Al-Shammary, A. H. A. Deciphered Entity of Extended Spectrum Beta Lactamase Resistant Staphylococcus aureus (ESβL) from Cows Mastitis Raw Milk Ecosystem in Baghdad Iraq. Plant Archives, 2020a, 20 (2): 1-20.
29. Razooqi, M. A. and Al-Shammary, A. H. A. Prevalence of forbidden contaminant clones of azoles and polyenes resistant Candida albicans in butter-cream ecosystem in Baghdad, Iraq. Plant Archives, 2020a, 20 (2): 40-50.
30. Zhang, C.M.; Xu, L.M.; Wang, X.C.; Zhuang, K. and Liu, Q.Q. Effects of ultraviolet disinfection on antibiotic-resistant Escherichia coli from wastewater: inactivation, antibiotic resistance profiles and antibiotic resistance genes. J. App. Microbiol., 2017, 123: 295—306.
31. Turnau, K.; , Fiałkowska, E.; Wazny, R.; Rozpadek, P.; Tylko, G.; Bloch, S.; Falenczyk, B. N.; Grabski, M.; Wegrzyn, A. and Wegrzyn, G. Extraordinary Multi-Organismal Interactions Involving Bacteriophages, Bacteria, Fungi, and Rotifers: Quadruple Microbial Trophic Network in Water Droplets. Int. J. Mol. Sci., 2021, 22 (2178): 1-24.
32. van de Sande, W. W. J.; Lo-Ten-Foe, van Belkum, J. R. A.; Netea, M. G.; Kullberg, B. J. and Vonk, A. G. Mycoviruses: future therapeutic agents of invasive fungal infections in humans? Eur J Clin Microbiol Infect Dis., 2010, 29: 755–763.
Received: January 15, 2023 / Accepted: February 25, 2023 / Published:15 March 2023
Citation. Al-Shammary A, Mounam M A. Exodia phenomenon of foodborne Mycophages cocktails against chimeric strains of Candida albicans recovered from dairy chain ecosystems in Baghdad. Revis Bionatura 2023;8 (1) 91 http://dx.doi.org/10.21931/RB/2023.08.01.91