2023.08.03.127

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Effect of spraying water on the body of Holstein cows during the dry period before calving under heat stress conditions in milk production and components.

Mohammed Ahmad Shwayel, Qais Abdul Jalil Al-Othman and Manar Abdul Qadir Al-Khafaji

1. College of Agriculture, University of Diyala [email protected].

2. station manager

3. College of Agriculture, University of Diyala, mm267414 @gmail.com.

* Correspondence: [email protected].

Available from. http://dx.doi.org/10.21931/RB/2023.08.03.127

ABSTRACT

The current experiment aims to reduce heat stress on pregnant imported Holstein cows in central Iraq during August and September. Used 12 multiparas Holstein dry (pregnant) cows raised At the Great Khalis cattle station in Hibhib sub-district in Diyala governorate and their ages were between third and fourth lactation similar in production and the pregnant cows were divided randomly into three treatments, each treatment containing 4 dry pregnant cows, treatment C cows without spraying (control treatment), treatment R1 Sprinkle the body with water 14 days before calving daily at 1 pm, treatment R2 Cows are spread a month before calving daily at 1 pm. The results of the statistical analysis showed that there were no significant differences between the treatments in the daily milk production, but an arithmetic increase was observed in the average daily milk production for the fifth, sixth and seventh month after birth for the treatment of spraying a month before calving, which recorded 18.25, 18.50 and 15.00 kg/cow/day Compared with the control treatment, which recorded a decrease in milk production 14.00, 15.00 and 12.50 kg/cow/day respectively, as well as no significant differences between treatments in total milk production during the experiment, but a Arithmetically increase was noted in the treatment spraying one month before calving which amounted to 944.28 kg/cow compared to the control treatment which recorded 812.32 kg/cow, As for the components of milk no significant differences were observed among the treatments after two months of birth, but discrepancies were noted among the treatments after 4 months of delivery, as the spraying treatment a month before calving recorded a significant increase in lactose and non-fat solids, which recorded 4.32 and 7.93 % compared with the control treatment of Without spray, which recorded 3.82 and 7.00%, respectively, and after 6 months of birth, the percentage of non – fat solids : protein, lactose and ash in the spray treatment a month before calving recorded a significant increase, as it was 2.77, 4.22 and 0.60% compared with the control treatment, which recorded 2.50, 3.77 and 0.52%, respectively. We conclude from this experiment that spraying cows before month calving improves the milk components significantly and the production in an arithmetical way.

Keywords: spray water, dry period, Holstein cow, heat stress

INTRODUCTION

Stress is a reflexive reaction that results in the animal's inability to adapt to its environment, which leads to many adverse effects, starting from the stresses facing the animal, causing a decrease in its production, leading to death 1, and heat stress is the most Harmful as it affects animal production. 2,3, represented by a set of environmental factors represented in temperature, relative humidity, air movement and solar radiation that cause the effective temperature of the environment to be higher than the comfort zone of cows 4. Studies have shown that cows exposed to heat stress during prenatally reduce postpartum milk production and composition 5,6,7, and an economic analysis indicated that stress caused economic losses in the United States estimated at $810 million annually ⁸. Exposing dry cows to heat stress before calving reduced udder growth after calving ⁹ and showed lower secretory activity and lower productivity after calving ¹⁰, thus producing less milk from 3 - 4.1 kg/cow/day compared with cows that were not exposed to heat stress during the prenatal period ¹¹^,12. The bad environmental conditions during the summer negatively affected the composition of cow's milk, as the milk contained a much lower percentage of fats, proteins, lactose and minerals ¹³. The use of water spray before calving during the dry period would reduce the adverse effects of heat stress, improve performance after birth, and reduce heat pressure on the growth of the mammary gland and milk production in the future, as some improvement was observed in milk production with cooling during the last three weeks before birth ¹⁴. Studies have shown that spraying cows during the dry period is essential for optimal udder growth, which affects milk production later; it was noted that heat stress delays the cellular renewal of the udder, which leads to a decrease in the proliferation of epithelial cells later in the dry period when there is a way to cool the body ⁹^,¹⁵. The current study aims to mitigate the negative impact of heat stress on pregnant imported Holstein cows in central Iraq.

MATERIALS AND METHODS

The experiment was carried out in the Great Khalis Cows Station in the Hibhab District - Khalis District in Diyala Governorate, which is 5 km away from the city, during the period from 1/8/2021 - 1/4/2022 using 12 Holstein cows from dry (pregnant) cows raised in the station. Their ages were between 7-5 years. Pregnant cows were divided into three treatments, each treatment containing 4 multiparous pregnant cows, as the treatments were C = non-sprayed cows (comparison treatment), R1 = water sprayed cows 14 days before calving and daily at 1 pm, R2 = water sprayed cows a month before calving and daily at 1 pm. The cows were placed in semi-misleading pens that contained feeders for the cow to eat from, and they had an iron clip designed so that the cow's head entered the trough to eat the fodder. The barn floor is a concrete cast, and vaccinations are given by specialized veterinarians at the station through periodic follow-up and monitoring of cows and conducting regular checks to ensure that they are free of diseases, especially mastitis. Pregnant non-milk-producing (dry period) cows were fed a mixture of concentrated fodder with feed materials available at the station. The grass is provided daily in the morning and evening, where the percentage of concentrated feed for pregnant cows was 8-6 kg/day and hay (dry, rough forage), either green grass or jet, is not given to cows that have two weeks left in their calving. After birth, brain fodder is provided because it contains phosphorous, which allows for a balance between phosphorous and calcium in the grass. After all, the fodder contains calcium and adding bran is an attempt to balance this ratio between them. Pregnant cows were sprayed daily during August and September; it was sprayed at 1 pm when temperatures were high, and the cow's entire body was sprayed with water (complete body washing). As the cows are entered through a corridor designed for spraying through tubes containing small holes with a firm thrust similar to a shower, the cows pass through which the whole body is washed from top to bottom. This room is called the sprayer. The air temperature was measured with an electronic thermometer of German origin that measures temperature and humidity at the same time. In the location of dry cows in the barn, the reading was recorded at 3 am and 3 pm daily for the duration of the experiment, and based on it, the Temperature-Humidity Index (THI) was calculated according to the method of Mader ¹⁷. One studied characteristic is milk production, as the cows are milked twice daily at 4 am and 4 pm using automated milking machines. The cows' parking space allows greater freedom and flexibility for the milker where he can easily see the cow's udder. After the cow's milking is completed, the milk produced is recorded with an electronic scale. This process is carried out for each cow daily (morning and evening) throughout the days of the experiment. milk components of the milk analyzer, as 100 ml of the milk sample was placed in a sealed bottle for each cow and transferred to the apparatus in the Abu Ghraib dairy factory in Baghdad to conduct the examination The milk was cooled and placed in a refrigerator at an appropriate temperature for the examination the next Day. The milk was cooled and placed in a refrigerator at an appropriate temperature for the examination the next Day Short on the device screen as percentages of each component of milk, which includes fat, protein, lactose, total solids and milk density.

Statistical analysis

CRD was used to analyze the results using SPSS ¹⁸, and the significant differences between the means were compared with the Duncan ¹⁹ multi-range test.

Moreover, using the following mathematical model:

Yij = µ+Ti +eij

Where:

Yij = the value of the view j of the treatment I

µ= the general average of the trait studied.

Ti the effect of treatment i.(spraying with water).

Eij= random error that is usually distributed.

Statistical analysis

Experimental data were analyzed according to a Completely Randomized Design, and the significant differences among means were detected using Duncan's Multiple Range Test at the probability of 0.05 ²². The linear model:

Yij = µ + τ_i + ε_ij

RESULTS

Table 1. Showed there were no Significant differences between the treatments in milk production, but arithmetically, increases were made in the fifth, sixth and seventh month for spray treatment month before calving, which recorded 18.25, 18.50 and 15.00 kg/cow/day Compared with the control treatment which recorded a decrease in milk production 14.00, 15.00 and 12.50 kg/cow/day. In contrast, the spray treatment 14 days before recorded 15.00, 16.00 and 12.00 kg/cow/day.

Table 1. Effect of spraying pregnant (dry) cows with water on the body before calving during the heat stress period of August and September in daily milk production (kg/cow/day) for the months after birth (mean ± standard error)

Moreover, it is noted in Table 2 that there were no Significant differences between the treatments in total milk production. However, there was an arithmetical increase in the spraying treatment one month before calving, which amounted to 944.28 kg/cow compared with the control treatment, which recorded 812.32 kg/cow, and the spray treatment before 14 days of calving, recorded 961.11 kg/cow.

Table 2. Effect of spraying pregnant (dry) cows with water on the body before calving during the heat stress period of August and September in Total milk production (kg/cow/day) for the seventh months after birth (mean ± standard error)

As for the components of milk, the results of the statistical analysis after two months of birth showed that there were no significant differences in the treatments for a percentage of milk fat after two months of birth, and after 4 months of birth, no significant effect was observed. However, an increase was noted in the spray treatment 14 days before birth where it recorded 5.08% and The spraying treatment before one month recorded 4.62% compared to the treatment without spraying which recorded 4.52%. It was not recorded after 6 months of birth any significant effect.

As for the protein in the milk of cows subjected to heat stress, it is noticed from the statistical analysis after two months of birth that there was no significant effect of the two treatments of spraying 14 days before and spraying a month before birth, where the percentage amounted to 2.90 % and 2.82%, respectively compared to the control treatment, which amounted to 2.62%, no significant differences were recorded after 4 months of birth. There was also an arithmetical increase in the spraying treatment a month before, which recorded 2.82%, compared to the treatment without spraying, which recorded 2.52%. In contrast, in the treatment of spraying 14 days before, it was recorded at 2.67%, and it was noted that there was a significant effect after 6 months of birth for the treatment. Spraying a month ago recorded 2.77%, and the treatment of spraying 14 days before and recorded 2.62%, compared with the treatment without spraying, which recorded 2.50%.

It was noted that the percentage of lactose sugar in milk were no significant differences among the treatments after two months of birth as it was recorded in the two treatments of spraying 14 days before birth and spraying one month before birth at 4.52% and 4.30% respectively. The control treatment was 4.02%, and it was recorded after 4 months of birth that there was a significant effect for the spray treatment a month before it recorded 4.32% compared to the control treatment which scored 3.82%. The spray treatment before 14 days recorded 4.05%, as well as 6 months after birth it was noted that there was a significant effect on the percentage of lactose sugar where the spray treatment recorded 4.22% before a month compared to the control treatment It amounted to 3.77%.

The results showed that two months after birth, there were no significant differences among the treatments in the percentage of ash in the milk, as the two treatments of spraying 14 days before birth and spraying a month before birth after two months of birth were 0.60% and 0.66% respectively. The ratio in the control treatment was 0.55%. There were no significant differences between the treatments for the percentage of ash in milk after 4 months of birth and after 6 months. A significant increase for the spraying treatment a month before, which recorded 0.60%, compared with the non-spraying treatment, which recorded 0.52%.

As for the non-fat solids in the milk of Holstein cows subjected to heat stress, the results showed that two months after birth, there were no significant differences among the treatments, and after 4 months of birth, a significant increase in the percentage of non-fat solids was observed in the spray treatment one month before calving, which amounted to 7.93% Compared with the control treatment which recorded 7.00%, and the spraying treatment was recorded before 14 days, 7.47%. However, after 6 months of birth, no significant differences were also observed among the treatments. As for the density of milk, it is noticed from the statistical analysis after two and four months of birth that there are no significant differences among the treatments for the studied trait, as well as after 6 months of birth.

Table 3. Effect of spraying pregnant (dry) cows with water on the body before calving during the heat stress period of August and September in milk components for 2 months after birth (mean ± standard error)

Table 4. Effect of spraying pregnant (dry) cows with water on the body before calving during the heat stress period of August and September in milk components for 4 months after birth (mean ± standard error)

Table 5. Effect of spraying pregnant (dry) cows with water on the body before calving during the heat stress period of August and September in milk components for 6 months after birth (mean ± standard error)

DISCUSSION

It was agreed in this study with previous studies, but the differences in this experiment were arithmetic, as previous studies indicated that when spraying with water on the body of cows during the period of heat stress as a means of cooling in the dry period, one month before birth, the production of birth milk improved by 1.4 kg / Day after birth ^14,19. Another study indicated that cows that did not experience heat stress before calving produced more milk after parturition ²⁰. A study indicated ⁹ that the milk production of sprayed cows was 33.9 kg/cow/day compared to cows not exposed to body spray, which recorded 28.9 kg/cow/day.

The absence of statistically significant differences between treatments in milk production for cows may be due to the high standard error in each treatment due to the small number of replicates for each treatment, which led to the absence of statistically significant differences between the treatments.

Researchers ^21,6 reported that the exposure of dry cows to prenatal spraying increased some milk components, with a protein content of 3.01% after parturition compared to dry, unsprayed cows, which scored 2.87% after parturition, and that the use of spray. It resulted in a 4.92% higher lactose compared to 4.67% control cows ²². Perhaps the reason for improving some milk components from the high percentages of some milk components in cows whose bodies were sprayed with water a month before giving birth may be because the spraying reduced the negative effect of heat stress on milk. Cows during that period ¹⁶ increased the activity and growth of udder secretory cells ¹⁵, which was reflected in the improvement of some milk components.

CONCLUSIONS

It is clear from previous experience that spraying water on pregnant Holstein cows imported a month before calving in heat stress has a positive role in improving and increasing some components of milk, which are protein, lactose and ash, and it also has a role in increasing milk production but arithmetically.

REFERENCES

1. Manuja B, Manuja A, Aich P..Stress and its impact on farm animals .2012. Frontiers in Bioscience; E4:1759-1767.

2. Rivington M, Matthews KB, Buchan K, Miller D, Russell G. Investigating climate change impacts and adaptation options using integrated assessment methods.2009 . Aspects of Applied Biology;93:85-92.

3. Roth Z. Influence of heat stress on reproduction in dairy cows physiological and practical aspects. 2020. Journal of Animal Science 98:80-87.

4. Alaaraji, S. F. T.; Awad, M. M.; Ismail, M. A. Study the Association of Asprosin and Dickkopf-3 with KIM-1, NTpro-BNP, GDF-15 and CPP among Male Iraqi with Chronic Kidney Disease. Systematic Reviews in Pharmacy 2020, 11 (5).

5. Tatar , A.; Kasaeizadeh, . R. .; Shobeirinia, B. .; Abdali, . L. .; Baeelashaki, R. . The Effect Of Using Different Levels Of Vitamin E On The Productive Traits Of Broiler Chickens. JLSAR 2023, 4, 30-36.

6. Tao, S., Monteiro, A.P., Thompson, I.M., Hayen, M.J., Dahl, G.E. Effect of late gestation maternal heat stress on growth and immune function of dairy calves. 2012. J. Dairy. Sci. 95: 7128–7136.

7. Othman Ghazi Najeeb Alani , Yassen Taha Abdul-Rahaman and Thafer Thabit Mohammed. Effect Of Vêo® Premium and Vitamin C Supplementation on Lipid Profile Before and During Pregnancy in Some Local Iraqi Ewes During Heat Stress. Iraqi Journal of Science.2021, Vol. 62, No. 7, pp: 2122-2130.

8. Ferreira FC, Gennari RS, Dahl GE, De Vries A. Economic feasibility of cooling dry cows across the United States. .2016.Journal of Dairy Science 99:9931-9941. doi:10.3168/jds.2016-11566.

9. Tao S, JW Bubolz, BC Do Amaral, IM Thompson, MJ Hayen, SE Johnson, GE Dahl.Effect of heat stress during the dry period on mammary gland development. 2011. Journal of dairy science. 94 (12) :5976-5986

10. Fabris, T.F., Laporta, J., Skibiel, A.L., Dado-Senn, B., Wohlgemuth, S.E., Dahl, G.E. Effect of heat stress during the early and late dry period on mammary gland development of Holstein dairy cattle. 2020. J. Dairy Sci. 103: 8576-8586.

11. Karimi, M.T., Ghorbani, G.R., Kargar, S., Drackley, J.K. Late-gestation heat stress abatement on performance and behavior of Holstein dairy cows. 2015. J. Dairy Sci. 98: 6865–6875.

12. Ibraheem M W, Muhaimeed A R, Mohammed Th. T. Leg cuts from Awaasi lambs fed a diet with varying levels of Rhus coriaria L., Physical dissection and chemical composition. Revis Bionatura 2022;7(4) 4. http://dx.doi.org/10.21931/RB/2022.07.04.4.

13. Imrich .Ivan, A,B,D–H , Róbert Toman1, A,B,H , Martina Pšenková1, C,G .Eva Mlyneková1, C,F,G , Tomáš Kanka1, C,E,G , Juraj Mlynek1, A,B , Barbora Pontešová1. Effect of temperature and relative humidity on the milk production of dairy cows.2021.Sci.12(1):22-27.

14. Urdaz, J. H., M. W. Overton, D. A. Moore, and J. E. P. Santos. Effects of adding shade and fans to a feedback sprinkler system for periparturient cows on health and performance. 2006. J. Dairy Sci. 89:2000–2006.

15. Wohlgemuth, S.E., Ramirez-Lee, Y., Tao, S., Monteiro, A.P.A., Ahmed, B.M., Dahl, G.E. Short communication: Effect of heat stress on markers of the mammary gland during the dry period. 2016. J. Dairy Sci. 99: 4875-4880.

16. Mader, T. L. ; L. J. Johnson. and J. B. Gaughan. A comprehensive index for assessing environmental stress in animals. 2006. J. of Anim. Sci. 88: 2153-2165.

17. SPSS. Statistical Package for Social Science version 20 for Windows LEAD Technologies. 2011. Inc. USA

18. Duncan, D. B. Multiple range and multiple F test. 1955. Biometrics 11:1-42.

19. Ali, H. H.; AL-Rawi, K.; Khalaf, Y.; Alaaraji, S.; Aldahham, B.; Awad, M.; Al-ani, O.; Al-ani, F.; Ali, A. T. Serum Caveolin-1 Level Is Inversely Associated with Serum Vaspin, Visfatin, and HbA1c in Newly Diagnosed Men with Type-2 Diabetes. Rep Biochem Mol Biol 2022, 11 (2).

20. Mellado, Miguel and Rodríguez-Godina, Iris Jazmin and García, José Eduardo and Morales, Juan Luis and Contreras.2022. During the Dry Period on Milk Yield and Reproductive Performance of Holstein Cows. .Pages (21) 22(5).Available at SSRN : https://ssrn.com/abstract=4038062 or http://dx.doi.org/10.2139/ssrn.4038062.

21. Noaman A I, Khalaf R M, Emad GH, Al-Abbasy, Mohammed Th. T. Effect of flaxseed oil dosing on fertility, growth characteristics and some physical, biochemical, and hormonal blood parameters during the early pregnancy of Awassi ewes. Revis Bionatura. 2022;7(4) 5. http://dx.doi.org/10.21931/RB/2022.07.04.5.

22. Martinez.RocioSoledad,RafaelAlejandeoPalladino,GeorggetBanchero,Rafael Fernandez-Martin,MarianaNanni,NicolasJuliano, Jesicaloio,Alejandro La Manna. Providing heat-stress abatement to late-lactation Holstin cows affects hormones ,metabolite blood proiles,and hepatic gene expression but not productive responses. 2021.Applied Animal Science 37(4) : 490-503.

Received: 25 June 2023/ Accepted: 26 August 2023 / Published:15 September 2023

Citation: Shwayel, M.and Al-Khafaji. Effect of spraying water on the body of Holstein cows during dry period before calving under heat stress conditions in milk production and components. Revis Bionatura 2023;8 (3) 127 http://dx.doi.org/10.21931/RB/2023.08.03.127